Investigation of The Relationship between The Pesticide Fluopyram and Parkinson’s disease

Parkinson's disease (PD) is a neurodegenerative defined as shaky stroke. It clinically characterized by; resting tremor, cogwheel rigidity, bradykinesia, and postural reflex impairment. also pathologically by Lewy bodies (LBs) formed the loss of dopaminergic neurons in Substantia nigra pars compacta (SNpc) region brain accumulation AlphaSynuclein (α-Syn) proteins. Pesticides are hugest risk factor PD. They cause formation PD using different mechanisms. permanently disrupt function electron transport complex (ETC) located mitochondria. Researches show that pesticide Rotenone (ROT) causes vivo vitro conditions inhibiting mitochondrial I causing oxidative stress. Fluopyram (Flu) frequently used toxicity like ROT. When literature searched, it makes one think about connection with seems there no available study on Flu fungicides utilized Isparta city at high rate for all planting types. The II inhibitor. inhibition complexes main pathway mechanism, therefore inhibitor may lead to same result. In this context, we created model mice ROT compare result be sure whether or not. Swiss albino male female were testing animals our study. Positive control (ROT Parkinson model, 1 mg/kg/day), negative (Solvent only, DMSO), (0.5,1 2 mg/kg/day) administrated daily doses subcutaneously (SC). experiment got completed after 21 days. Motor Function Test, Histochemical Immunohistochemical Studies, Comet assay, quantitative real-time PCR (qRT-PCR) well. results, statistically significant changes observable animal weights groups. motor test, decrease group was observable, On other hand, detected specifically value which applied doses. tissues belong tested groups examined, all-important structural such LBs observed SN. belonging examined α-Syn immunohistochemical staining, more positive markings observable. Tyrosine hydroxylase (TH) fewer observed. has been found DNA damage blood tissues. determined do not have damage. increase SNCA mRNA expression levels while decreasing TH DJ-1 levels. Comparing increased but significant. With detection decreased levels, difference shown results. conclusion, results proved Flu's induce phenotype depends recommended research especially methods